Dino

Hoechst Mycoplasma Stain Kit

Hoechst Mycoplasma Stain Kit

Packaging size: 1 Kit

SKU 093030000
Price:
$639.40
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Description

Product Description

For the Detection of Mycoplasma in Cell Culture Reliable - uses Hoechst stain method Versatile - detects mycoplasma AND bacteria, yeast, fungi Rapid - takes less than 2 hours Economical - 1

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Detection of mycoplasma

Specifications
SKU 093030000
Hazard Statements H317
Applications Detection of mycoplasma
Materials Required Not Supplied 1. Sterile distilled water for dilution of 10X HBSS. 2. Incubator (37 °C) 3. Refrigerator 4. Various glassware 5. Fluorescent microscope 6. Carnoy's fixative or 1 part glacial acetic acid to 3 parts absolute methanol.
Pack Size 1 Kit
Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Handle with gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Kit with 4 Components
Presentation Kit with 4 Components
Research Areas Immunostaining and Detection||Immunostaining and Detection||Microbiological Analysis||Contamination, Prevention, Detection & Removal
Specificity This stain is highly specific for DNA. It is thought to occupy one of the grooves of the DNA double helix; binding by intercalation. The stain will cause cellular nuclei to fluoresce; however; the other major DNA cytoplasmic components - mitochondria - will not. All prokaryotic organisms (Mycoplasmas; bacteria; yeast; fungi) will give off fluorescence. The identification of the contaminant must be made by noting size; morphology and relationship to the cell.
Storage and Handling Concentrated Hoechst stain; Hanks Balanced Salt Solution; slides and mounting medium may be stored at 2 °C to 8 °C or below; Store kit at +4 °C.
Typical Working Concentration The Hoechst Stain should give maximum fluorescent intensity when diluted 1:10 with Hanks Balanced Salt Solution, pH 7.0, achieving a final concentration at 0.05 ug/mL.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.