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BCIP/NBT, liquid substrate, 100 mL
(5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting procedures only. Concentration: 0.577 mmol/liter BCIP; 0.122 mmol/liter NBT.
TMB (3,3',5,5' Tetramethylbenzidine)
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.