Proteins & Enzymes
Streptavidin (from Streptomyces avidinii) is conjugated with highly purified alkaline phosphatase (calf intestine) by a modified glutaraldehyde method. The typical working dilution is 1:500-1:1250 for immunoblotting, 1:1000-1:12500 for ELISA, and 1:20-1:50 for immunohistochemistry. 2 ml.
Bovine Albumin 30% Solution Polymer Enhanced
30% Polymer Enhanced Solution
Prepared from high quality Fraction V Powder intended for in vitro diagnostic use in immunohematological procedures requiring high protein diluent. It can be used for serological procedures, and it potentiates the expression of weak antigen-antibody reactions and avidity of red blood cells.
Cellulase Y-C (From Trichoderma Viride)
Cellulase Y-C enzyme retains very high filter paper decomposing activity and showed an appreciable amount of hemicellulase. Actually, this enzyme removed cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.
Proline Specific Endopeptidase (From Flavobacterium Meningosepticum)
Proline Specific Endopeptidase specifically cleaves peptide bonds on the carboxy side of proline residues. Much slower hydrolysis is observed on the carboxy side of alanine residues. This enzyme is very close, in its properties, to a post-proline cleaving enzyme. The substrates have been found to have the general structure Y-Pro-X, where Y is a peptide or N-protected amino acid and X may be an amino acid, peptide, amide or ester.
Human Albumin Fraction V
This human protein is prepared by a modification of the Cohn procedure. Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis). Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.