Rabbit IgG Fraction To Human Albumin

Product is the lyophilized powder of rabbit IgG fraction to human albumin and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation

SKU	0855029
Apply Add To Quote	No
Antibody Type	Polyclonal Antibody
Application Notes	This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining.
Base Catalog Number	55029
Concentration	Total Protein: 15.1 mg/mL; Antibody Protein: 5 mg/mL
Conjugate	Unconjugated
Formulation Details	Lyophilized
Host	Rabbit
Immunogen	Human albumin
Pack Size	2 mL
Preparation Method	The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity	Human
Specificity	It shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Sterility	Filtered through a .22 µm filter
Typical Working Concentration	Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA/FIA
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.