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Anti-human IgG Fc goat IgG fraction, fluorescein-conjugated, 2 mL
Product Description
Fluorescein-Conjugated Goat IgG Fraction To Human IgG Fc is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human IgG Fc and buffer salts.
Application Notes
Fluorescein-Conjugated Goat IgG Fraction To Human IgG Fc is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Specifications
| SKU | 0855150 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-Conjugated Goat IgG Fraction To Human IgG Fc is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals). |
| Base Catalog Number | 55150 |
| Concentration | Total Protein: 35.1 mg/mL; Antibody Protein: 5 mg/mL |
| Conjugate | FITC |
| Formulation Details | Lyophilized |
| Host | Goat |
| Immunogen | Purified human IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. |
| Purity | Purified |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human, IgG Fc |
| Specificity | This product shows reactivity to human Fc and IgG; cross-reactivity to other species may exist. Antibody activity to human Fab; IgA; IgM; or non-immunoglobulin serum proteins is not present. |
| Sterility | Filtered through a .22 µm filter |
| Typical Working Concentration | Dilution Reccomondations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations
