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Anti-human lambda Light chain (Bound) goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-human lambda Light chain (Bound) goat IgG fraction, fluorescein-conjugated, 2 mL

$330.90

Key features and details

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human lambda light chain (bound) and buffer salts.

SKU: 0855160

Packaging size:
2 mL
Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human lambda light chain (bound) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855160
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Base Catalog Number 55160
Concentration Total Protein: 11.2 mg/mL F/P Ratio: 5.86 mol/mol
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, Lambda Light Chain (Bound)
Specificity This product shows reactivity to bound lambda light chains; cross-reactivity to other species may exist. Because the product is directed against the lambda chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to free lambda light chains may be present. Antibody activity to kappa Fab; free kappa light chains; or non-immunoglobulin serum proteins is not present.
Typical Working Concentration DILUTION RECCOMENDATIONS: 1:100 - 1:400 for Tissue Staining 1:250 - 1:1,000 for Immuno Blotting 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Citations