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Anti-human IgG goat F(Ab')2 fragment, fluorescein-conjugated (whole molecule), 2 mL
Product Description
Fluorescein-conjugated Goat F(ab')2 fragment to Human IgG is the fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human IgG (whole molecule) and buffer salts.
Application Notes
Fluorescein-conjugated goat F(ab')2 fragment to human IgG is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. The antibody may also be used in FACS-based assays (1:200).
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
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Datasheet
Datasheet
Brochures
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Specifications
| SKU | 0855180 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-conjugated goat F(ab')2 fragment to human IgG is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies and their F(ab')2 fragments are recommended to avoid non-specific binding from the inherent antibodies of host animals. The antibody may also be used in FACS-based assays (1:200). |
| Base Catalog Number | 55180 |
| Concentration | Total Protein: 28.1 mg/mL; Antibody Protein: 10 mg/mL |
| Conjugate | FITC |
| Formulation Details | Lyophilized |
| Host | Goat |
| Immunogen | Pooled normal human serum |
| Pack Size | 2 mL |
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer and vialed. |
| Purity | Purified |
| Solubility | Soluble in Water |
| Species Reactivity | Human (Whole Molecule) |
| Specificity | This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | Dilution Recommendations: Direct immunofluorescence (1:16); ANA-indirect immunofluorescence (1:32 or 1:64); FACS-based assays (1:200); 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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