Anti-human IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, fluorescein-conjugated, 1 mg

Product Description

Fluorescein-Conjugated Goat Affinity Purified F(ab')₂ Fragment to Human IgG (Whole Molecule) is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified F(ab')₂ fragments to human IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat Affinity Purified F(ab')₂ Fragment to Human IgG (Whole Molecule) is suitable for use in direct immunofluorescence, as well as for ANA (Anti-Nuclear Antibody)-indirect immunofluorescence. The antibody may also be used in FACS-based assays. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications

SKU	0855201
Apply Add To Quote	No
Antibody Type	Polyclonal Antibody
Application Notes	Fluorescein-Conjugated Goat Affinity Purified F(ab')₂ Fragment to Human IgG (Whole Molecule) is suitable for use in direct immunofluorescence, as well as for ANA (Anti-Nuclear Antibody)-indirect immunofluorescence. The antibody may also be used in FACS-based assays. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals; these F(ab')₂ fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number	55201
Concentration	Total Protein: 1.07 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate	FITC
Formulation Details	Liquid
Host	Goat
Immunogen	Pooled normal human serum
Pack Size	1 mg
Personal Protective Equipment	Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method	Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Purity	Affinity Purified
Solubility	Soluble in Water
Species Reactivity	Human (Whole Molecule)
Specificity	This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration	ANA (Anti-Nuclear Antibody)-indirect immunofluorescence: 1:32; Direct immunofluorescence: 1:16. Dilution recommended: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.