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Anti-human IgG (whole molecule) rabbit IgG fraction, peroxidase-conjugated , 2 mL
Product Description
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit IgG fraction to human IgG (whole molecule) and buffer salts.
Application Notes
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Datasheet
Datasheet
Specifications
| SKU | 0855221 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55221 |
| Concentration | Total Protein: 6.4 mg/mL Titer: 1:170;000 |
| Conjugate | HRP |
| Formulation Details | Lyophilized |
| Host | Rabbit |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. |
| Purity | Purified |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human (Whole Molecule) |
| Typical Working Concentration | DILUTION RECCOMENDATIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1:8,000 for Immuno Blotting 1:2,000 - 1:16,000 for ELISA/FIA |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations
