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Anti-bovine IgG (whole molecule) rabbit IgG fraction, fluorescein-conjugated, 2 mL
Product Description
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to bovine IgG (whole molecule) and buffer salts.
Application Notes
Immunoblotting
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Datasheet
Datasheet
Specifications
| SKU | 0855280 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Immunoblotting |
| Base Catalog Number | 55280 |
| Concentration | Total Protein: 15.5 mg/ml F/P Ratio: 4.2 mol/mol Antibody Protein: 5 mg/ml |
| Conjugate | FITC |
| Host | Rabbit |
| Pack Size | 2 mL |
| Solubility | Soluble in water |
| Species Reactivity | Bovine (Whole Molecule) |
| Specificity | The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. This product shows reactivity to bovine IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations