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Anti-dog IgG (whole molecule) rabbit IgG fraction, fluorescein-conjugated, 2 mL
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to dog IgG (whole molecule) and buffer salts.
Application Notes
FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Certificate of Analysis
Datasheet
Material Safety Datasheet
SKU | 0855326 |
Alternate Names | Anti-Dog |
Antibody Type | Polyclonal Antibody |
Application Notes | FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. |
Base Catalog Number | 55326 |
Concentration | Total Protein: 21.9 mg/ml F/P Ratio: 5.95 mol/mol Antibody Protein: 4 mg/ml |
Conjugate | FITC |
Formulation Details | Lyophilized |
Host | Rabbit |
Immunogen | Dog IgG |
Pack Size | 2 mL |
Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge |
Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. |
Purity | Purified |
Solubility | Soluble in water |
Species Reactivity | Dog (Whole Molecule) |
Specificity | This product shows reactivity to dog IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
Typical Working Concentration | Suggested working dilution: Direct immunofluorescence: 1:32 |
Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |