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Anti-mouse IgG (no cross anti-human) sheep IgG fraction, peroxidase-conjugated, 2 mL

Anti-mouse IgG (no cross anti-human) sheep IgG fraction, peroxidase-conjugated, 2 mL

$315.05
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to mouse IgG (no cross to human) and

SKU: 0855558

MDL Number:
MFCD00162644
Packaging size:
2 mL
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Product Description
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to mouse IgG (no cross to human) and buffer salts.

Application Notes

Peroxidase conjugated Sheep IgG fraction to mouse IgG is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). It may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. The product can also be used for direct ELISA.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855558
Antibody Type Polyclonal Antibody
Application Notes Peroxidase conjugated Sheep IgG fraction to mouse IgG is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). It may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. The product can also be used for direct ELISA.
Base Catalog Number 55558
Concentration Total Protein: 14.9 mg/mL; Titer: 1:187;000
Conjugate HRP
Formulation Details Lyophilized
Host Sheep
Immunogen Purified mouse IgG
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Purity Immunoaffinity Chromatography 
Solubility Soluble in water
Species Reactivity Mouse (No Cross To Human)
Specificity Product shows reactivity to mouse IgG. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to human IgG or mouse non-immunoglobulin serum proteins is not present.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Datasheets

Datasheets
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United States)