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Anti-mouse IgG (whole molecule) goat affinity-purified antibody, peroxidase-conjugated , 2 mL
Product Description
Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.
Application Notes
Peroxidase-conjugated goat affinity purified antibody to mouse IgG is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.) Anti-mouse IgG (whole molecule)-peroxidase antibody can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
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Certificate of Analysis
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Datasheet
Datasheet
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Specifications
| SKU | 0855563 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-conjugated goat affinity purified antibody to mouse IgG is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.) Anti-mouse IgG (whole molecule)-peroxidase antibody can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis. |
| Base Catalog Number | 55563 |
| Concentration | Total Protein: 0.7 mg/ml Titer: 1:48;000 |
| Conjugate | HRP |
| Formulation Details | Liquid |
| Host | Goat |
| Immunogen | Purified mouse IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Mouse (Whole Molecule) |
| Specificity | Product shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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