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Anti-rabbit IgG (whole molecule) goat IgG fraction, 5 mL
Product Description
Product is the lyophilized powder of goat IgG fraction to rabbit IgG (whole molecule) and buffer salts.
Application Notes
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | ELISA | Immunohistochemistry | Immunoblot
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Datasheet
Datasheet
Brochures
Brochures
Specifications
| SKU | 0855622 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals). |
| Base Catalog Number | 55622 |
| Concentration | Total Protein: 26.5 mg/mL; Antibody Protein: 12 mg/mL |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized |
| Host | Goat |
| Pack Size | 5 mL |
| Preparation Method | The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Solubility | Soluble in water |
| Species Reactivity | Rabbit (Whole Molecule) |
| Specificity | It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | Suggested working dilutions: 1:50 - 1:200 for Tissue Staining, 1:100 - 1:500 for Immunoblotting, 1:400 - 1:2,000 for Elisa |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations
