Anti-rabbit IgG (whole molecule), peroxidase-conjugated, 2 mL

Product Description

Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts. Antiserum is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from goat anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

Application Notes

Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications

SKU	0855689
Antibody Type	Polyclonal Antibody
Application Notes	Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number	55689
Concentration	Total Protein: 0.8 mg/ml Titer: 1:20;000
Conjugate	HRP
Formulation Details	Purified
Host	Goat
Pack Size	2 mL
Preparation Method	Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added.
Purity	Affinity Purified
Solubility	Soluble in water
Species Reactivity	Rabbit (Whole Molecule)
Specificity	It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration	SUGGESTED WORKING DILUTIONS: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 - 1:4,000 for Elisa
Usage Statement	Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.