Join the Fight, Find the Cure! Get 20% OFF Cancer & Life Science products. Use promo code: SAVE20LFS at the checkout. Offer ends February 26th, 2021.
In Stock
Anti-rabbit IgG Fc affinity-purified goat antibody, peroxidase-conjugated, 2 mL
Product Description
Product is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG Fc and buffer salts.
Application Notes
This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.)
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
Documents
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Certificate of Analysis
Datasheet
Datasheet
Specifications
| SKU | 0855690 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.) |
| Base Catalog Number | 55690 |
| Concentration | Total Protein: 0.6 mg/ml Titer: 1:23;000 |
| Conjugate | HRP |
| Formulation Details | Purified |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | IgG Fc, Rabbit |
| Specificity | It shows reactivity to rabbit Fc and IgG; cross-reactivity to other species may exist. Antibody activity to F(ab')<sub>2</sub>; IgA; IgM; or non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | SUGGESTED WORKING DILUTIONS: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 -1: 4,000 for Elisa |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations