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Anti-rabbit IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, peroxidase-conjugated, 1 mL

Anti-rabbit IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, peroxidase-conjugated, 1 mL

$209.05
Peroxidase-conjugated goat affinity purified F(ab')2 fragment to Rabbit IgG (Whole Molecule) is horseradish peroxidase (HRP)-conjugated goat affinity

SKU 0855693

MDL Number
MFCD00162788
Packaging size
1 mL
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Product Description
Peroxidase-conjugated goat affinity purified F(ab')2 fragment to Rabbit IgG (Whole Molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified F(ab')2 fragments to rabbit IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855693
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Base Catalog Number 55693
Concentration Total Protein: 1.0 mg/mL; Titer: 1:20;000
Conjugate HRP
Formulation Details Purified
Host Goat
Immunogen Purified rabbit IgG
Pack Size 1 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rabbit (Whole Molecule)
Specificity Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution. Peroxidase-conjugated goat anti-rabbit IgG antibody was used for wester blot analysis of yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)

Datasheets

Datasheets
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United Kingdom)