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Anti-rat IgG (whole molecule) goat IgG fraction, peroxidase-conjugated, 2 mL

Anti-rat IgG (whole molecule) goat IgG fraction, peroxidase-conjugated, 2 mL

$396.30
Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction

SKU 0855770

Packaging size
2 mL
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Product Description
Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855770
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Base Catalog Number 55770
Concentration Total Protein: 5.5 mg/mL Titer: 1:120;000
Conjugate HRP
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Purity Purified
Solubility Soluble in water
Species Reactivity Rat (Whole Molecule)
Specificity It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1;8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Datasheets

Datasheets
English (United States)