Anti-ß-galactosidase goat IgG fraction (no cross to E. coli), 1 mL

Product Description

Product is goat IgG fraction to ß-galactosidase (no cross to E. coli) and buffer salts.

Application Notes

Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation

Specifications

SKU	0856028
Apply Add To Quote	No
Antibody Type	Polyclonal Antibody
Application Notes	Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
Base Catalog Number	56028
Concentration	Total Protein: 31.1 mg/mL; Antibody Protein: 3 mg/mL; Conc/Titer: 1:50-1:200 Tissue Stain; 1:100-1:500 Immunoblot; 1:400-1:2;000 ELISA; conc is at 3 mg/mL
Conjugate	Unconjugated
Formulation Details	Liquid
Host	Goat
Immunogen	Recombinant full length protein of <i>Escherichia coli</i> β Galactosidase.
Pack Size	1 mL
Preparation Method	The antibody is produced by hyperimmunizing goats with the enzyme β-galactosidase from <i>Escherichia coli</i>. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-β-galactosidase <i>E. coli</i> proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.
Solubility	Soluble in water
Specificity	It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of <i>E. coli</i>.
Typical Working Concentration	Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa
Uniprot Number	SwissProt: P00722 <i>Escherichia coli</i>
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.