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Anti-goat IgG (whole molecule) affinity-purified rabbit antibody,alkaline phosphatase-conjugated

Anti-goat IgG (whole molecule) affinity-purified rabbit antibody,alkaline phosphatase-conjugated

MDL Number: MFCD00162335

SKU 0859292-CF
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Description

Product Description

Product is alkaline phosphatase (AKP)-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts.

Application Notes

Alkaline phosphatase-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections). Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody can be used in immunoblotting and ELISA. Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Immunoblot | Immunohistochemistry

Specifications
SKU 0859292-CF
Application Notes Alkaline phosphatase-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) is suitable for use in immunoblotting. The product can also be used for direct ELISA (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections). Detection of TLR2 and TLR4 in protein extracts from epidermal keratinocytes was performed by western blot using alkaline phosphatase conjugated rabbit anti-goat IgG as the secondary at a 1:2500 dilution. Polyclonal anti-goat IgG (whole molecule)–alkaline phosphatase antibody can be used in immunoblotting and ELISA. Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Base Catalog Number 59292
Concentration Conjugate Protein: 0.6 mg/mL
Conjugate ALK
Formulation Details Liquid
Host Rabbit
Immunogen Purified goat IgG
Preparation Method Antibody and highly purified calf-intestine alkaline phosphatase are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by gel filtration. The product is dialyzed into 0.01M TRIS-HCl, 0.25M sodium chloride, pH 8.0, with 15 mg/mL bovine albumin and 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.
Purity Affinity Purified
Solubility Soluble in water
Specificity It shows reactivity to goat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.