Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.

Application Notes

Tryptic Soy Agar is used for the isolation and cultivation of fastidious and non-fastidious micro-organisms. It supports the abundant growth of fastidious organisms such as Pneumococci, Streptococci, Neisserias, etc. Since it lacks carbohydrates, it is very useful in the study of hemolytic reactions and also in the preparation of chocolate agar. Also, it may be used for sterility testing. It can be used with or without blood supplement. It is used as precultivation medium and enumeration (E. coli) acc. to membrane-filter technique. As it does not contain the X and V factors, it is suitable for identification of Haemophilus sp. by adding X (Hemin) and V (DPN) factors strips.

Key Applications

Bacteria Cell Culture | Isolation and cultivation of fastidious organisms.