McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki.

Application Notes

1× Park and Terasaki's Modification Of McCoy 5A is used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures. It is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and transformed cell types, and the most demanding primary and continuous cell lines.

Key Applications

Mammalian Cell Culture | Production of virus in primary cell cultures