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GENECLEAN® Kit, 200 Preps

GENECLEAN® Kit, 200 Preps

$165.60

Key features and details

  • For the rapid purification of DNA from TAE and TBE agarose gels
  • Optimal purification of 200 bp to 20 kb DNA
  • Each spin column can bind up to 10 μg of DNA
  • Kit can also be used to purify DNA from polyacrylamide gels

SKU: 111001200

Synonyms
DNA Clean-Up Kit
Sample Volume:
Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Packaging size:
200 Preps
Product Description

The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.

Application Notes

The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications
SKU 111001200
Alternate Names DNA Clean-Up Kit
Application Notes The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.
Base Catalog Number 1001200
Brand GENECLEAN®
Components NaI| New Wash Concentrate| Glassmilk™| BioFloat™ floating incubation rack
Format Geneclean® slurry
Pack Size 200 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH NAI pH Range: 7.0-7.4| Geneclean New Wash pH Range: 7.4 - 7.7
Preparation Method Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Recommended Alternate GENECLEAN® II Kit| GENECLEAN® III Kit| GENECLEAN® SPIN Kit| GENECLEAN® Turbo Kit
Safety Symbol GHS05, GHS06
Sample Source Gels and Solutions
Sample Type Enzymatic Reactions, Solutions, TAE buffered agarose gels
Sample Volume Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Unit Definition 1 μl of GLASSMILK™ will bind 1-2 μg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Citations