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GNOME® DNA Isolation kit

GNOME® DNA Isolation kit

Synonyms DNA Purification

SKU 112010400-CF
$133.00
In Stock
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Description

Product Description

The GNOME® DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells. Each preparation with the GNOME® kit yields up to 100 µg of genomic DNA. The DNA isolated by the GNOME¨ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.

Application Notes

The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Isolation of high molecular weight DNA from a variety of sources

Specifications
SKU 112010400-CF
Alternate Names DNA Purification
Application Notes The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
Base Catalog Number 2010400
Components Cell Lysis/Denaturing solution | RNase Mixx | Protease Mixx | Salt out Mixture | Cell Suspension solution.
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Cell Lysis/Denaturing Solution: pH range= 6.2-6.9 |Salt-out Mixture: pH range= 6.0-6.8 |Cell Suspension Solution: pH range= 8.0-8.2
Preparation Method Sample preparation protocols provided in kit insert. The yield of genomic DNA and the speed of the protocol are greatly enhanced through the use of the FastPrep®System.
Safety Symbol GHS05 |GHS07 |GHS08
Sample Volume 20-50 mg wet weight bacterial cell pellet | up to 250 mg plant tissue |2-5 x 107 nucleated white blood cells |50-250 mg mammalian tissue |~1-5 x 108 yeast cell pellet
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Format All necessary reagents