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Alkali-Cation™ Yeast Transformation Kit, 250 preps
Product Description
Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes this the method of choice for the high efficiency transformation needed in two hybrid screens.
Application Notes
Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Transformation of yeast cells
Specifications
| SKU | 112200200 |
| Alternate Names | High Efficiency Yeast Transformation Kit |
| Application Notes | Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies. |
| Base Catalog Number | 2200200 |
| Components | Sterile TE Buffer pH 7.5 |Lithium/Cesium Acetate |Carrier DNA 10 mg/mL Sterile |Sterile Solution Histamine |TE/Cation MIXX |PEG |Sterile Solution SOS |
| Format | All necessary reagents |
| Pack Size | 250 Preps |
| Personal Protective Equipment | Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves. Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Recommended Alternate | EZ-Yeast™ Transformation Kit |
| Safety Symbol | GHS07 |GHS08 |
| Sample Type | Overnight yeast culture or colonies from plates |
| Sample Volume | 500 µL or 3-4 mm colony |
| Unit Definition | ≥ 104 transformants/µg plasmid DNA |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
Citations