2XYT-broth agar, capsules

2XYT-broth agar, capsules

Synonyms Bacteriophage Media

SKU 113012231-CF
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Description

Product Description

YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.

Application Notes

2xYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant Escherichia coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture, Cloning, Gene expression, protein expression, Proteomics, Bacterial Culture, Genetic Manipulation, Plasmid Production, Purification and Detection, Recombinant Protein Expression and Analysis, Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Specifications
SKU 113012231-CF
Alternate Names Bacteriophage Media
Application Notes 2xYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant Escherichia coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.
Base Catalog Number 3012231
Formulation Details Contents Per Liter: 16 g Tryptone-B; 10 g Yeast Extract-B; 5 g NaCl; 15 g Agar-B
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.9 ± 0.3 at 25°C.
Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in 2xYT-Broth formulations. Chosen reagents are combined in the proper proportions according to the 2xYT-Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 46 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).
Solubility Soluble in water (46 g/L).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Format Capsules
Applications Cell culture, Cloning, Gene expression, protein expression, Proteomics, Bacterial Culture, Genetic Manipulation, Plasmid Production, Purification and Detection, Recombinant Protein Expression and Analysis, Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
Media Type 2xYT

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