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3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

$694.30

Key features and details

3,3,5,5-Tetramethylbenzidine Liquid Substrate

SKU: 0219602591

Synonyms
TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
CAS Number:
54827-17-7
Molecular Weight:
240.35 g/mol
Packaging size:
1 L
Product Description

3,3,5,5-Tetramethylbenzidine Liquid Substrate

Application Notes

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Substrate for horseradish peroxidase

Documents

Datasheet

Datasheet
Specifications
SKU 0219602591
Alternate Names TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
Application Notes TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Base Catalog Number 2196025
CAS # 54827-17-7
Format Ready-to-use solution, Stabilized
Hazard Statements H226-H302-H312-H332-H370
Melting Point 334 to 336° F (NTP, 1992)
Molecular Weight 240.35 g/mol
Pack Size 1 L
Safety Symbol GHS02, GHS07, GHS08
Solubility less than 1 mg/mL at 68° F (NTP, 1992)
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Citations