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3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L
3,3,5,5-Tetramethylbenzidine Liquid Substrate
Application Notes
TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Substrate for horseradish peroxidase
Datasheet
SKU | 0219602591 |
Alternate Names | TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System |
Application Notes | TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity. |
Base Catalog Number | 2196025 |
CAS # | 54827-17-7 |
Format | Ready-to-use solution, Stabilized |
Hazard Statements | H226-H302-H312-H332-H370 |
Melting Point | 334 to 336° F (NTP, 1992) |
Molecular Weight | 240.35 g/mol |
Pack Size | 1 L |
Safety Symbol | GHS02, GHS07, GHS08 |
Solubility | less than 1 mg/mL at 68° F (NTP, 1992) |
Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |