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3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

3,3,5,5-Tetramethylbenzidine liquid substrate, 1 L

$694.30
3,3,5,5-Tetramethylbenzidine Liquid Substrate

SKU: 0219602591

Synonyms
TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
CAS Number:
54827-17-7
Molecular Weight:
240.35 g/mol
Packaging size:
1 L
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Product Description
3,3,5,5-Tetramethylbenzidine Liquid Substrate

Application Notes

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Substrate for horseradish peroxidase

Specifications
SKU 0219602591
Alternate Names TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
Application Notes TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Base Catalog Number 2196025
CAS # 54827-17-7
Format Ready-to-use solution, Stabilized
Hazard Statements H226-H302-H312-H332-H370
Melting Point 334 to 336° F (NTP, 1992)
Molecular Weight 240.35 g/mol
Pack Size 1 L
Safety Symbol GHS02, GHS07, GHS08
Solubility less than 1 mg/mL at 68° F (NTP, 1992)
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.