AlbumiNZ™ low endotoxin BSA, stem cell grade, ≥97%

AlbumiNZ™ low endotoxin BSA, stem cell grade, ≥97%

CAS Number: 9048-46-8 Molecular Weight: 66KDa

EC Number: 232-936-2

SKU 02199896-CF
In Stock
Request a quote
Description

Product Description

Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. It contains 17 intrachain disulfide bridges and 1 sulfhydryl group

Application Notes

Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Bovine albumin contains 16% nitrogen and is often used as a standard in protein calibration studies. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell culture applications, Immunoassay, Stem cell research

Specifications
SKU 02199896-CF
Application Notes Albumin binds water, Ca2+, Na+, and K+. Due to a hydrophobic cleft, albumin binds fatty acids, bilirubin, hormones and drugs. The main biological function of albumin is to regulate the colloidal osmotic pressure of blood and to a lesser degree to provide cellular nutrition. Bovine albumin contains 16% nitrogen and is often used as a standard in protein calibration studies. Albumin is used to solubilize lipids, stabilize protein solutions, and is also used as a blocking agent in Western blots or ELISA applications.
Base Catalog Number 199896
Biochemical Physiological Actions Albumin loses its helical structure rapidly in 2-4 M urea and 4 M guanidinium chloride. The unfolding is reversible, even, to some extent, after concomitant reduction of all disulfide bonds . Reduced albumin can reform into structures capable of binding antibodies and other ligands . Formation of SS bonds is rapid but the return of native structure is slow and some wrong disulfide pairings may persist . After oxidation of the cystine bridges, however, native structure is irreversibly lost.
CAS # 9048-46-8
Concentration Total Protein: ≥95%
EC Number 232-936-2
Molecular Weight 66KDa
pH 6.5 - 7.5 (4% aq soln)
Purity ≥97% Total Protein
Solubility Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of albumin is very good (especially if the solutions are stored aliquoted and frozen). Albumin is readily coagulated by heat. When heated to 50 °C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. Dissolves completely in 20 min at 20-25 °C (20%).
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Applications Cell culture applications, Immunoassay, Stem cell research