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Albumin, bovine, fraction V, protease free
Albumin is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Immunoblot | ELISA |Mammalian Cell Culture |ADME| Standards
|Alternate Names||BSA; Bovine albumin|
|Application Notes||Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.|
|Base Catalog Number||820221,820224,820225,820226|
|Formulation Details||Powder |Protease Free|
|Isoelectric Point||At 25 °C: Fatty Acid Depleted- 5.3, Endogenous Material- 4.7; 4.9 (Lit.)|
|pH||7.0 (10% aqueous solution); 6.9 (saline)|
|Preparation Method||Plasma undergoes salt fractionation using caprylic acid (sodium salt), heating at 65 °C for three hours; alcohol precipitation followed by an acetone wash resulting in a Fraction V powder|
|Protein or Enzyme Type||Albumins|
|Solubility||Soluble in 40 mg/mL, H<sub>2</sub>O.|