Thrombin, bovine, low specific activity

Thrombin, bovine, low specific activity

Synonyms Factor IIa; Thrombin from bovine plasma

CAS Number: 9002-04-4

EC Number: 232-648-7

SKU 02199907-CF
$148.00
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Description

Product Description

Thrombin is used throughout the diagnostics industry in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls. Thrombin is also used for site specific cleavage of recombinant fusion proteins and in biochemical and medical research applications.
Bovine thrombin, low specific activity, is manufactured in New Zealand from bovine plasma of New Zealand origin using prothrombin activated with thromboplastin extracted from bovine lung tissue. Low specific activity Thrombin is lyophilized under vacuum in glass vials, or in closed trays as a bulk powder, depending on customer requirements. Low specific activity thrombin has a specific activity of 90-300 NIH units/mg protein and greater than 50% total protein.

Application Notes

Thrombin is used throughout the diagnostics industry in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls. Thrombin is also used for site specific cleavage of recombinant fusion proteins, and in biochemical and medical research applications. Bovine Thrombin, low specific activity, is manufactured from New Zealand-sourced bovine plasma using prothrombin activated with thromboplastin extracted from bovine lung tissue. Thrombin is lyophilized in glass vials under vacuum to optimize product performance. Low specific activity thrombin has 90 - 300 NIH units/mg and greater than 50% total protein. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.

Usage Statement

Low specific activity thrombin is to be used for Research or Diagnostics Use Only. Not to be used for in-vivo therapeutic uses, or in any type of consumption or applications for human or animal use.

Specifications
SKU 02199907-CF
Alternate Names Factor IIa; Thrombin from bovine plasma
Application Notes Thrombin is used throughout the diagnostics industry in a variety of coagulation assays, clotting factor tests and defibrination of blood or plasma for serum controls. Thrombin is also used for site specific cleavage of recombinant fusion proteins, and in biochemical and medical research applications. Bovine Thrombin, low specific activity, is manufactured from New Zealand-sourced bovine plasma using prothrombin activated with thromboplastin extracted from bovine lung tissue. Thrombin is lyophilized in glass vials under vacuum to optimize product performance. Low specific activity thrombin has 90 - 300 NIH units/mg and greater than 50% total protein. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.
Base Catalog Number 199907
Biochemical Physiological Actions Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
CAS # 9002-04-4
EC Number 232-648-7
Extinction Coefficient E<sub>280</sub><sup>1%</sup> = 18.3 (human); E<sub>280</sub><sup>1%</sup> = 19.5 (bovine)
Hazard Statements H315-H319-H334-H335
pH 7.0 - 8.0
Protein or Enzyme Type Plasma & Blood Proteins
RTECS Number XO8950000
Safety Symbol GHS07, GHS08
Specific Activity 90 - 300 u/mg protein
Unit Definition Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37 °C.
Usage Statement Low specific activity thrombin is to be used for Research or Diagnostics Use Only. Not to be used for in-vivo therapeutic uses, or in any type of consumption or applications for human or animal use.

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