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Bovine Transferrin and Applications

Iron is an essential growth nutrient in almost all living organisms and is toxic in its free form. It must thus be carried by proteins such as Transferrin, which has the capacity to bind ferric ions. Transferrin is hence considered the most ‘natural’ means of managing iron transport.


Transferrin is the major serum protein produced in the liver and secreted into the blood. It is responsible for the transport of iron in almost all cell types. In cell culture applications, Transferrin is used to control iron metabolism. It is also used as a nutrient in cell and microbial culture and as a media supplement in the production of biopharmaceutical products.

A specialized manufacturer of high-quality biological products, MP Biomedicals New Zealand Limited (MP Bio NZ) has gained an international reputation for providing the highest quality bovine plasma proteins to the biopharmaceutical, animal health, diagnostic industries and research institutes.

MP Bio NZ uses chromatographic techniques for separation and purification of its proteins, as opposed to the traditional methods of cold ethanol (Cohn) fractionation and BSA stabilized heat shock. This technologically advanced process leaves the protein molecules fully intact and, in turn, leads to a higher cell culture and cell expression in bioprocessing applications. Higher cell growth and production titers results in substantial savings in terms of time and process efficiencies, thereby leading to lower costs.

The manufacturing facility is located in Auckland, New Zealand. It is equipped with world-class equipment and has a team of industry experts who can respond quickly to customer expectations.

MP Bio New Zealand offers 2 forms of Transferrin viz:

  • Holo-transferrin - Saturated with iron Fe3+ and used to supply iron in cell culture media

  • Apo-transferrin - free of iron and used to remove iron in cell culture media

  • Both forms of Transferrin are subjected to pasteurization (10 hr at 60 ◦C)

  • Extracted using chromatographic methods

 

Primary applications of Transferrin:

  • Aids in prevention of iron toxicity in mammalian cell culture systems

  • Assists in controlling iron metabolism in cell culture systems

  • An integral part of serum-free or reduced serum media

  • Used in bio-manufacturing and tissue culture

 

Bovine Immunoglobulin G from MP Biomedicals New Zealand   

 

NZ Bovine Transferrin (Holo), Heat Treated (SKU: 02152335)

Bovine Transferrin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. Transferrin (HOLO) is an iron-rich product supplied as a heat-treated, lyophilised powder.

Bovine Transferrin (HOLO) is frequently used as a nutrient in cell and microbial culture and as a media supplement in bio-pharmaceutical production. Bovine holo-transferrin can be used with a wide range of cell types.

Key features and details

  • Purified from New Zealand-sourced bovine plasma
  • ISO quality assuring complete traceability and consistent high quality
  • Iron-rich product supplied as a heat-treated, lyophilized powder
 

NZ Bovine Transferrin (Apo), Heat Treated (SKU: 02152334)

Bovine Transferrin is an iron-depleted product supplied as a heat-treated, lyophilised powder.

Bovine Transferrin (APO) is frequently used as a nutrient in cell and microbial culture and as a media supplement in bio-pharmaceutical production. It is a useful transport factor in cell culture work to convey essential metabolites to the cells. Important factor when culturing cells in a defined medium.

All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.

     

Product specifications

Test HOLO Transferrin (Cat.# 02152335) APO Transferrin (Cat.# 02152334)
Appearance Red to Brown Powder Off-white to Pink
Bioburden (CFU/g) ≤ 100 ≤ 100
Endotoxin (EU/mg) ≤ 1 ≤ 1
Iron (μg/g protein) ≥ 1200 ≤ 40
Moisture by Karl Fischer, w/w ≤ 5% ≤ 5%
Mycoplasma None Detected None Detected
pH 6.0 – 8.0 7.0 – 8.0
Purity (SDS-PAGE), w/w Total Protein ≥ 95% ≥ 95%
Solubility (3% w/v in water) Dissolves in ≤ 20 minutes at 20 - 25 o C Dissolves in ≤ 20 minutes at 20 - 25 o C
Total Protein, anhydrous
- Kjeldahl Nitrogen, w/w
≥ 95% ≥ 95%
Virus 9CFR 113.53(c) Not Detected Not Detected

 

MP Bio New Zealand Proteins are Chromatographically Extracted from Bovine Plasma under Quality Systems that are ISO Certified and Audited to cGMP Principles.

All products are manufactured using New Zealand sourced raw materials. Documentation standards at MP Bio NZ are of the highest order and can support the exacting standards that are often sought by the pharmaceutical industry, which operates in a highly regulated environment.

MP Biomedicals has an ideal option for your requirements. Contact us to learn more about our full array of services.

 

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