Equine anti-globulin (Coombs' Test) reagent, 2 mL

Product Description

The anti-globulin or Coombs' test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia. Antibody alone or in the presence of complement injures the cell membrane permitting leakage of RBC material, allowing recognition by the reticuloendothelial cells, thereby leading to phagocytosis and destruction of RBC's. Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal

Application Notes

Equine anti-Globulin reagent has been widely used for the diagnosis of autoimmune hemolytic anemia (AIHA) in a variety of species. Although not common AIHA has been reported in the horse. Equine anti-globulin reagent is also useful in the neonatal isoerythrolysis test. The anti-globulin procedure has also been used in equine blood typing.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Immunoassays | Autoimmune Hemolytic Anemia (AIHA) diagnosis

Specifications

SKU	08646371
Antibody Type	Polyclonal Antibody
Application Notes	Equine anti-Globulin reagent has been widely used for the diagnosis of autoimmune hemolytic anemia (AIHA) in a variety of species. Although not common AIHA has been reported in the horse. Equine anti-globulin reagent is also useful in the neonatal isoerythrolysis test. The anti-globulin procedure has also been used in equine blood typing.
Base Catalog Number	646371
Conjugate	Unconjugated
Disease Screened	Autoimmune hemolytic anemia (AIHA)
Formulation Details	Lyophilized
Host	Rabbit
Pack Size	2 mL
Preparation Method	Antibodies are produced in rabbits by immunization with equine immunoglobulins and formulated with selected β globulins including the C3 component of complement. The resulting antiserum is heated at 56 °C for 30 minutes to inactivate rabbit complement. Heteroagglutinins are absorbed with pooled normal equine red blood cells. The reagent is tested with normal and antibody-coated equine red blood cells to assure its specificity.
Solubility	Soluble in water
Species Reactivity	Horse
Usage Statement	Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.