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Ethidium bromide solution, 10 mL

Ethidium bromide solution, 10 mL

$62.25
Ethidium Bromide Solution

SKU 04802511

Synonyms
3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI); Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI); 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phenylphenanthridine ethobromide; Dromilac; Homidium bromide
CAS Number
1239-45-8
Molecular Formula
C21H20BrN3
Molecular Weight
394.316 g/mol
Beilstein Registry Number
3642536
EC Number
214-984-6
MDL Number
MFCD00011724
Packaging size
10 mL
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Product Description
Ethidium Bromide Solution

Application Notes

Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Ready for CE IVD labeling of clinical applications.

Key Applications

Electrophoresis;Staining

Specifications
SKU 04802511
Alternate Names 3,8-Diamino-5-ethyl-6-phenylphenanthridiniumbromide (6CI); Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide(8CI,9CI); 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium bromide; 2,7-Diamino-9-phenylphenanthridine ethobromide; Dromilac; Homidium bromide
Application Notes Ethidium bromide(EtBr) has been used extensively to reduce mitochondrial DNA copy number in proliferating cells and most commonly used in nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Base Catalog Number 802511
Beilstein Registry Number 3642536
Biochemical Physiological Actions Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
CAS # 1239-45-8
Density 0.34 g/cm³
EC Number 214-984-6
Flash Point >100 °C
Format Solution
Melting Point 260-262 deg C (decomposes)
Molecular Formula C21H20BrN3
Molecular Weight 394.316 g/mol
Pack Size 10 mL
RTECS Number Ethidium Bromide SF7950000
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a> is 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 2 mg/mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Ready for CE IVD labeling of clinical applications.
Vapor Pressure 1.2X10-12 mm Hg at 25 deg C /Estimated/
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