Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. This protein is prepared by a modification of the Chen low pH charcoal method. Contains < 0.1 mg/g fatty acids. The venous blood from which this product is manufactured has been tested for the presence of Hepatitis B Surface Antigen (HBsAg) and found to be non-reactive by a currently approved FDA test. The blood has also been tested for the presence of Anti-HCV, Anti-HIV-1 and Anti-HIV-2, antibodies and found to be negative. The source donors have been tested in accordance with 21 CFR 640. This blood is non-reactive for syphilis.

Application Notes

Albumin may be used to eliminate background interference in ELISA's or other enzyme assay systems. It is also used in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells. Human and bovine albumins contain 16% nitrogen and are often used as standards in protein calibration studies. Due to their free hydrophobic region fatty acid free albumins are used to solubilize lipids in tissue culture, and are also used as blocking agents in Western blots or ELISA applications. Globulin free albumins are suitable for use in applications where no other proteins should be present (e.g., electrophoresis).

Key Applications

Immunoassays | Mammalian Cell Culture