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Trypan blue, 0.4% solution in PBS, 100 mL

Trypan Blue is a blue acid dye with a strong affinity for cellulose. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

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