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Alkali-Cation™ Yeast Transformation Kit, 250 preps
This yeast transformation kit provides the reagents needed to perform an easy and quick transformation of yeast cells. Can be used to transform linear DNA into P. pastoris for protein expression studies.
Tryptic soy broth, 500 g
Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum.
RPMI 1640 (1X) w/o L-glutamine and L-Leucine, 100 mL
RPMI 1640 medium is a general purpose medium based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.
Pluronic® F-68 polyol, 100 g
Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.
Dulbecco's Modified Eagle's Medium (1X Solution) with 4.5 g/L dextrose, w/o L-glutamine and inositol, 500 mL
Also known as DME, Dulbecco's Modified Eagle's Medium is the most widely used modification of Eagle's Basal Medium (BME).