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PIPES, cell culture reagent, 1 kg
Product Description
Pipes
Application Notes
PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Zwitterionic Buffer
Documents
Datasheet
Datasheet
Specifications
| SKU | 0219455291 |
| Alternate Names | Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperazine-N,N; 1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperzine-N,N'-bis(ethanesulfonic acid) |
| Application Notes | PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. |
| Base Catalog Number | 19455291 |
| Beilstein Registry Number | 817713 |
| CAS # | 5625-37-6 |
| EC Number | 227-057-6 |
| Format | Powder |
| Grade | Cell Culture Grade |
| Molecular Formula | C8H18N2O6S2 |
| Molecular Weight | 302.36 g/mol |
| Pack Size | 1 kg |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| pKa | 6.8 at 25 °C; 6.66 at 37 °C(Lit.) |
| Sterilization of Solutions | it is recommended to filter sterilize using a 0.2 um filter. |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| UV Visible Absorbance | OD260 (0.1M in 0.2M NaOH): ≤0.05; OD280 (0.1M in 0.2M NaOH): ≤0.05 |
Citations