PIPES, cell culture reagent, 1 kg

Product Description

Pipes

Application Notes

PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Zwitterionic Buffer

Specifications

SKU	0219455291
Alternate Names	Piperazine-N,N´-bis[2-ethanesulfonic acid]; Piperazine-N,N; 1,4-Piperazinediethanesulfonic acid; Piperazine-1,4-bis(2-ethanesulfonic acid); Piperzine-N,N'-bis(ethanesulfonic acid)
Application Notes	PIPES is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Base Catalog Number	19455291
Beilstein Registry Number	817713
CAS #	5625-37-6
EC Number	227-057-6
Format	Powder
Grade	Cell Culture Grade
Molecular Formula	C8H18N2O6S2
Molecular Weight	302.36 g/mol
Pack Size	1 kg
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
pKa	6.8 at 25 °C; 6.66 at 37 °C(Lit.)
Sterilization of Solutions	it is recommended to filter sterilize using a 0.2 um filter.
Usage Statement	Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance	OD260 (0.1M in 0.2M NaOH): ≤0.05; OD280 (0.1M in 0.2M NaOH): ≤0.05