Product is the lyophilized powder of rabbit IgG and buffer salts. Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.

Application Notes

Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis