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Tris-glycine buffer, 1 box
Product Description
Tris-Glycine Buffer
Application Notes
Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Used for Western blotting and gel electrophoresis
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Specifications
SKU | 04816200 |
Application Notes | Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. |
Base Catalog Number | 816200 |
CAS # | Not applicable |
Components | 0.025 M Tris, 0.192 M Glycine |
Format | Powder |
Pack Size | 1 box |
Personal Protective Equipment | Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter |
Preparation Method | Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use. |
Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |
Citations