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Product Description
Tris-Glycine Buffer
Application Notes
Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used for Western blotting and gel electrophoresis
Specifications
| SKU | 04816200 |
| Apply Add To Quote | No |
| Application Notes | Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose. |
| Base Catalog Number | 816200 |
| CAS # | Not applicable |
| Components | 0.025 M Tris, 0.192 M Glycine |
| Format | Powder |
| Pack Size | 1 box |
| Personal Protective Equipment | Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter |
| Preparation Method | Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
Documents
![MP COA [816200] lot ID: 1716A](https://qa.mpbio.com/static/version20220119100957Z/frontend/Mpbio/default/en_US/images/document.png "MP COA [816200] lot ID: 1716A")