Tris-Glycine Buffer

Application Notes

Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Used for Western blotting and gel electrophoresis

Datasheet

DS [04816200]

Material Safety Datasheet

AE_EN

SKU	04816200
Application Notes	Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
Base Catalog Number	816200
CAS #	Not applicable
Components	0.025 M Tris, 0.192 M Glycine
Format	Powder
Pack Size	1 box
Personal Protective Equipment	Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Preparation Method	Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use.
Usage Statement	Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Tris-glycine buffer, 1 box